The major museums of the island are:
● Archaeological Museum. Located near the port. Among the exhibits is a letter of Alexander the Great to the people of Chios. Also excavation findings from Emporios, Kato Fana, Agio Gala and others.
● Byzantine museum. Located on the central square "Vounaki”. Old Ottoman mosque.
● Museum Ioustiniani. Located in the Castle of Chios, near the main gate. On exhibit: Byzantine frescos, Post-Byzantine icons, Byzantine sculptures.
● The Korai Library (one of the largest in Greece), inside which the Argenti Museum of Ethnology and Folklore is housed.
● The Naval Museum. It covers an important gap in the maritime history of the island.
● The Castle of Chios. It was built by the Byzantines as a fortress and it was extended by the Genoese in the 14th century. When the Turks conquered Chios in the 16th century, they added other buildings inside the Castle.
● The Monastery of Agios Mina, where in 1822, during the Massacre of Chios, over 3000 people were slaughtered inside the monastery by the Turks. Today the monastery is a national monument.
● Nea Moni (New Monastery) with the Byzantine mosaics. Built in the 11th century by the Byzantine Emperor Constantine IX, Monomachos. One of the most beautiful Byzantine monasteries in Greece.
● "Citrus, memory scent”. A modern museum, which refers to Kampos and in particular the cultivation of citrus fruit and the wealth they brought to the island's economy.